Then, select the appropriate post-hoc method to use. Recommended.‘ option is selected, found under the ‘ Multiple comparisons test‘ header. Ensure the ‘ Correct for multiple comparisons: Confidence intervals and significance. Finally, click the ‘ Options‘ tab at the top of the window. This will enable post-hoc testing to be carried out to determine where, if any, the significance lies.ĥ. Select the ‘ Compare the mean of each column with the mean of every other column‘ option. Next, click the ‘ Multiple comparisons‘ tab at the top. Use ANOVA’ under the ‘ Assume Gaussian distribution’ header below.Ĥ. In this case, select the ‘ No matching or pairing’ option, under the ‘ Experimental design‘ header. The next window will ask you to specify which test to perform. Double check that the datasets are ticked on the right window to be included in the test. Select the ‘ One-way ANOVA (and nonparametric)’ analysis under the ‘ Column analyses‘ section. Here you need to tell GraphPad which test to perform.
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